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sds page zymogram miniprotean 3 cell  (Bio-Rad)
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Sds Page Zymogram Miniprotean 3 Cell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds page gelatin zymography  (Bio-Rad)
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Bio-Rad sds page gelatin zymography
Cytotoxicity of PAMs toward cultured cancer cell lines, HT-1080 and HL-60 cells. (A) Cytotoxicity of PAMs toward HT-1080 (solid bars) or HL-60 (lined bars) cells. The cytotoxicity was evaluated by using the CytoTox 96 kit (Promega) after 8 h of incubation with PAMαHL or PAMαHLG1; the absorbance at 490 nm representing the concentration of released lactate dehydrogenase was recorded with a microtiter plate reader. Cytotoxicity (%) = (( A s – A spon )/( A max – A spon )) × 100 where A s is the absorbance of toxin-treated cells, A spon is the absorbance of untreated cells (spontaneously dead cells), and A max is the absorbance of cells treated with lysis buffer. (B) Binding assay of PAMs to HT-1080 and HL-60 cells determined by electrophoresis in a 10% SDS-polyacrylamide gel, followed by autoradiography. PAMs (PLHL, PLHLG1, IGHL, IGHLG1; 62 nM) were incubated with HT-1080 cells or HL-60 cells (1 × 10 7 cells mL –1 ) in 5% CO 2 for 40 min at 37 °C. After centrifugation, the resuspended pellets and the supernatants were analyzed. ▲, oligomers; P, pellet; S, supernatant. (C) Extent of MMP-2 expression from HT-1080 and HL-60 cells analyzed by SDS-PAGE gelatin <t>zymography.</t> HT-1080 cells and HL-60 cells were incubated in serum-free DMEM and IMDM, respectively, in 5% CO 2 at 37 °C overnight. The media were collected, centrifuged to remove debris, and mixed with zymogram sample buffer before loading into the zymogram gel (2 μg final concentration of total protein).
Sds Page Gelatin Zymography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zymogram analyses sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page  (Bio-Rad)
97
Bio-Rad zymogram analyses sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page
Cytotoxicity of PAMs toward cultured cancer cell lines, HT-1080 and HL-60 cells. (A) Cytotoxicity of PAMs toward HT-1080 (solid bars) or HL-60 (lined bars) cells. The cytotoxicity was evaluated by using the CytoTox 96 kit (Promega) after 8 h of incubation with PAMαHL or PAMαHLG1; the absorbance at 490 nm representing the concentration of released lactate dehydrogenase was recorded with a microtiter plate reader. Cytotoxicity (%) = (( A s – A spon )/( A max – A spon )) × 100 where A s is the absorbance of toxin-treated cells, A spon is the absorbance of untreated cells (spontaneously dead cells), and A max is the absorbance of cells treated with lysis buffer. (B) Binding assay of PAMs to HT-1080 and HL-60 cells determined by electrophoresis in a 10% SDS-polyacrylamide gel, followed by autoradiography. PAMs (PLHL, PLHLG1, IGHL, IGHLG1; 62 nM) were incubated with HT-1080 cells or HL-60 cells (1 × 10 7 cells mL –1 ) in 5% CO 2 for 40 min at 37 °C. After centrifugation, the resuspended pellets and the supernatants were analyzed. ▲, oligomers; P, pellet; S, supernatant. (C) Extent of MMP-2 expression from HT-1080 and HL-60 cells analyzed by SDS-PAGE gelatin <t>zymography.</t> HT-1080 cells and HL-60 cells were incubated in serum-free DMEM and IMDM, respectively, in 5% CO 2 at 37 °C overnight. The media were collected, centrifuged to remove debris, and mixed with zymogram sample buffer before loading into the zymogram gel (2 μg final concentration of total protein).
Zymogram Analyses Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Sds Page, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds zymogram gels  (Thermo Fisher)
99
Thermo Fisher sds zymogram gels
Cytotoxicity of PAMs toward cultured cancer cell lines, HT-1080 and HL-60 cells. (A) Cytotoxicity of PAMs toward HT-1080 (solid bars) or HL-60 (lined bars) cells. The cytotoxicity was evaluated by using the CytoTox 96 kit (Promega) after 8 h of incubation with PAMαHL or PAMαHLG1; the absorbance at 490 nm representing the concentration of released lactate dehydrogenase was recorded with a microtiter plate reader. Cytotoxicity (%) = (( A s – A spon )/( A max – A spon )) × 100 where A s is the absorbance of toxin-treated cells, A spon is the absorbance of untreated cells (spontaneously dead cells), and A max is the absorbance of cells treated with lysis buffer. (B) Binding assay of PAMs to HT-1080 and HL-60 cells determined by electrophoresis in a 10% SDS-polyacrylamide gel, followed by autoradiography. PAMs (PLHL, PLHLG1, IGHL, IGHLG1; 62 nM) were incubated with HT-1080 cells or HL-60 cells (1 × 10 7 cells mL –1 ) in 5% CO 2 for 40 min at 37 °C. After centrifugation, the resuspended pellets and the supernatants were analyzed. ▲, oligomers; P, pellet; S, supernatant. (C) Extent of MMP-2 expression from HT-1080 and HL-60 cells analyzed by SDS-PAGE gelatin <t>zymography.</t> HT-1080 cells and HL-60 cells were incubated in serum-free DMEM and IMDM, respectively, in 5% CO 2 at 37 °C overnight. The media were collected, centrifuged to remove debris, and mixed with zymogram sample buffer before loading into the zymogram gel (2 μg final concentration of total protein).
Sds Zymogram Gels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cytotoxicity of PAMs toward cultured cancer cell lines, HT-1080 and HL-60 cells. (A) Cytotoxicity of PAMs toward HT-1080 (solid bars) or HL-60 (lined bars) cells. The cytotoxicity was evaluated by using the CytoTox 96 kit (Promega) after 8 h of incubation with PAMαHL or PAMαHLG1; the absorbance at 490 nm representing the concentration of released lactate dehydrogenase was recorded with a microtiter plate reader. Cytotoxicity (%) = (( A s – A spon )/( A max – A spon )) × 100 where A s is the absorbance of toxin-treated cells, A spon is the absorbance of untreated cells (spontaneously dead cells), and A max is the absorbance of cells treated with lysis buffer. (B) Binding assay of PAMs to HT-1080 and HL-60 cells determined by electrophoresis in a 10% SDS-polyacrylamide gel, followed by autoradiography. PAMs (PLHL, PLHLG1, IGHL, IGHLG1; 62 nM) were incubated with HT-1080 cells or HL-60 cells (1 × 10 7 cells mL –1 ) in 5% CO 2 for 40 min at 37 °C. After centrifugation, the resuspended pellets and the supernatants were analyzed. ▲, oligomers; P, pellet; S, supernatant. (C) Extent of MMP-2 expression from HT-1080 and HL-60 cells analyzed by SDS-PAGE gelatin zymography. HT-1080 cells and HL-60 cells were incubated in serum-free DMEM and IMDM, respectively, in 5% CO 2 at 37 °C overnight. The media were collected, centrifuged to remove debris, and mixed with zymogram sample buffer before loading into the zymogram gel (2 μg final concentration of total protein).

Journal: ACS Central Science

Article Title: Redirecting Pore Assembly of Staphylococcal α-Hemolysin by Protein Engineering

doi: 10.1021/acscentsci.8b00910

Figure Lengend Snippet: Cytotoxicity of PAMs toward cultured cancer cell lines, HT-1080 and HL-60 cells. (A) Cytotoxicity of PAMs toward HT-1080 (solid bars) or HL-60 (lined bars) cells. The cytotoxicity was evaluated by using the CytoTox 96 kit (Promega) after 8 h of incubation with PAMαHL or PAMαHLG1; the absorbance at 490 nm representing the concentration of released lactate dehydrogenase was recorded with a microtiter plate reader. Cytotoxicity (%) = (( A s – A spon )/( A max – A spon )) × 100 where A s is the absorbance of toxin-treated cells, A spon is the absorbance of untreated cells (spontaneously dead cells), and A max is the absorbance of cells treated with lysis buffer. (B) Binding assay of PAMs to HT-1080 and HL-60 cells determined by electrophoresis in a 10% SDS-polyacrylamide gel, followed by autoradiography. PAMs (PLHL, PLHLG1, IGHL, IGHLG1; 62 nM) were incubated with HT-1080 cells or HL-60 cells (1 × 10 7 cells mL –1 ) in 5% CO 2 for 40 min at 37 °C. After centrifugation, the resuspended pellets and the supernatants were analyzed. ▲, oligomers; P, pellet; S, supernatant. (C) Extent of MMP-2 expression from HT-1080 and HL-60 cells analyzed by SDS-PAGE gelatin zymography. HT-1080 cells and HL-60 cells were incubated in serum-free DMEM and IMDM, respectively, in 5% CO 2 at 37 °C overnight. The media were collected, centrifuged to remove debris, and mixed with zymogram sample buffer before loading into the zymogram gel (2 μg final concentration of total protein).

Article Snippet: MMP-2 enzymatic activity in tissue culture media was determined by 10% SDS-PAGE gelatin zymography (Ready Gel Zymogram Gel, Bio-Rad).

Techniques: Cell Culture, Incubation, Concentration Assay, Lysis, Binding Assay, Electrophoresis, Autoradiography, Centrifugation, Expressing, SDS Page, Zymography